M. Abd-Allah¹* and M. W. H. Daghash²

¹Department of Animal Production, Faculty of Agric. Al-Azhar Univ, Assiut Branch, Assiut, Egypt.

²Department of Animal Production, Faculty of Agric. Assiut Univ, Assiut, Egypt.

Abstract :

This research was conducted to elucidate the effect of flavomycin® and propolis powder,  as feed additives, on  milk yield of  buffalo and   productive performance  of suckling calves such as total body weight, average daily gain, body measurements and selective metabolic parameters.  For this reason, sixteen multiparous Egyptian buffalo with an initial body weight of 445±11.45 kg. and their offspring suckling calves (16 calves) were used in this experiment. The trial was started at 6 weeks prenatal pregnancy and 4 months after parturition (suckling period) till weaning. The animals (mother and their suckling calves) were divided into four equal groups (4 animals / each group) according the preceding lactation season. First group (CO) was fed on the diet without any additives. Second group (T1) was fed on the diet + 50 mg of propolis/head / day. Third  group (T2) was fed on the diet + 60 mg of flavomycin/ head/ day, while fourth group (T3) was fed on the diet + 50 mg of propolis + 60 mg flavomycin / head / day. The buffaloes were fed on a concentrated diet consisting of 45% seed, 15% cotton seed, 15% soy bean, 22% wheat bran, 1% premix salts, 1% limestone and 1% salt while suckling calves  were allocated with their mothers and fed colostrum  for the first three days.  Milk, starter and treatments were given to suckling calves till weaning. The amount of buffalo milk were estimated. Body weight, body measurements and blood samples were taken from newborn calves. Results revealed that productive performance and selective blood metabolites were affected by supplemented diets. Daily and total milk yield were increased insignificantly in animals fed treated diets than untreated ones. There was a significant improvement of daily and final body weight (P<0.05) in the suckling calves treated with propolis alone or combined with flavomycin until the weaning period.  Final chest girth, body high and abdominal circumference (AC) measurements were increased insignificantly for calves treated with propolis. There was a tendency of higher concentration of total protein in plasma of calves fed flavomycin (T2) than those fed control diets. Concentration of albumin was decreased insignificantly (P>0.05) in treated calves compared with control. Furthermore, flavomycin in the diets (T2) increased total globulin insignificantly (P>0.05) by about 29% compared with the control group. Treated diets induced the lowest levels in the plasma triglyceride and total cholesterol (P<0.01) in comparison to the control diets. Concentration of glucose was decreased (P<0.05) while transaminases enzyme levels were increased (P<0.05) in treated calves compared with untreated ones. It could be concluded that added flavomycin and /or propolis in the diets increased milk yield and improved live body weight and growth in suckling calves with   improvement in final chest girth, body high and abdominal circumference measurements. Selected blood plasma metabolites affected by dietary treated diets without any harmful effect on health of suckling claves.

المراجع :

Abdel-Hamid, A.M., E.I. Shehata and M.E. Ahmed (1999). Physionutritional studies on pregnant and lactating goats fed on rations differing in roughage /concentrate ratio at different feeding levels and or not supplemented with bentonite: Effects on the blood profile .J.Agric .Sci, Mansoura Univ., 24(9): 4587.

Abdel-Monemm, U.M. (2013). Effect of Dietary Supplementation of Flavomycin on the Growth Performance, Digestibility Traits, Carcass Characteristics, Carcass Meat Chemical Analysis and Some Blood Components of Lambs. Australian Journal of Basic and Applied Sciences, 7(1): 589-594.

Albokhadaim, I. (2015). Influence of dietary supplementation of propolis on hematology, biochemistry and lipid profile of rats fed high cholesterol diet. Journal of Advanced Veterinary and Animal Research, (2): 56–63.

Bankova V., R. Christov., M.C. Marcucci., S, Popov (1998). Constituents of Brazilian geopropolis. Z Naturforsch 53c: 402-406. 

Banskota, A.H., Y. Tezuka., I.K. Adnyana., K. Midorikawa., K. Matsushige.,D. Message., A.A. Huertas and S. Kadota (2000). Cytotoxic, hepatoprotective and free radical scav­enging effects of propolis from Brazil, Peru, the Netherlands and China. J. Ethnopharmacol., 72: 239-246.

Bauer, F. and G. Dost (1974). Flavomycin, the first antibiotic intended exclusively as a food supplement. Farbwerke Hoechst. AFrankfurta Main. Hoechst, 87-92.

Cafantaris, B. (1981). Uber die wirkung von antibiotika-zusatzenauf die mikrobielle garung im pansensaft in vitro (The effect of antibiotic additives on the microbial fermentation in rumen fluid in vitro). Agrar-Diss., Universitat Hohenheim.

Corlateanu, D. (1976). Treatment with bee products of neonatal calves, 2^nd Int. Symposium on Apitherapy, Bucharest, September 2^nd – 7^nd, Romania, 295-297.

Cottica, S.M., A.C.H.F. Sawaya., M.N. Eberlin., S.L. Franco., L.M. Zeoula., J.V. Visenteiner (2011). Antioxidant activity and composition of propolis obtained by different methods of extraction. J. Braz. Chem. Soc. 22, 929-935.

De Castro, S.L. (2001). Propolis: Biological and pharma­cological activities. Therapeutic uses of this bee product. Annu. Rev. Biomed. Sci., 3: 49-83.

Denli, M., S. Cankaya, S. Silici1., F. Okan and A.N. Uluocak (2005). Effect of Dietary Addition of Turkish Propolis on the Growth Performance, Carcass Characteristics and Serum Variables of quail (Coturnix coturnix japonica). Asian-Aust. J. Anim. Sci, 18(6):848-854.

Duncan, D.B.(1955). Multiple range and multiple F tests. Biometrics 11, 1–42.

Economides, S., E. Georghiades, Ch. Papachristoforou and M. Hadjipanayiotou (1988). The effect of feed antibiotics and of vitamin A on the performance of Chois lambs and Friesian calves. Technical Bulletin 101.

El-Ashry, M.A., Afaf, M. Fayed., K.M.Youssef., F.A. Salem and G.M. Abdul-Aziz (2003). Effect of feeding flavomycin or yeast as feed supplement on lambs' performance in Sinai. Egypt. J .Nutr. and Feeds,1009- 1022.

El-Basiony, A.Z. (1994). Performance of growing lambs and buffalo calves given flavomycin as feed supplement. Annuals of Agric. Sci., Moshtohor, 32(4):1511-1520.

El-Beshbishy, H.A., A.N. Singab., J. Sinkkonen and L. Pihlaja (2006). Hypolipidemic and antioxidant effect of Morus Alba L. (Egyptian mulberry) root bark fractions supplementations in cholesterol fed rats. Life Sc., (78): 2727-2733.

El-Sayed, M.E, O.M. Abo-Salem., H.A Aly, A.M. Mansour (2009). Potential Antidiabetic and Hypolipidemic effects of propolis extract in STZ-induced diabetic Rats. Pak, J. Pharm Sci., (22):168–74.

Galal, A., A.M.H. Ahmed., W.A.H. Ali., M.H. Elsanhoury., H.E. Ahmed (2008). Residual feed intake and its effect on cell-mediated immunity in laying hens given different propolis levels. International Journal of Poultry Science 7, 1105-1111.

Hafez, A.M., H. Ibrahim, A. Gomaa, A.A. Farrag and I.A. Salem (1983). Enzymatic and heamatological studies on buffalo's preparturient periods. Assiut Vet. Med. J., 11(21): 172.

Harper, H.A., V.W. Rodwell and P.A. Mayer (1977). Review of physiological chemistry, 6^th (Eds.) Lange Medical Publication, California.

Hassan, E.A.S. (2009). Utilization of growth promoters and bentonite in sheep rations. Ph.D Thesis, Faculty of Agriculture, Al-Azhar University.

Hegazi A.G. and F. Abd El-Hady (1996). Influence of administration of propolis on chicken immune status. The Egyptian Journal of Immunology, 3(1):111-116.

Heydari, K.H., N. Dabiri, J. Fayazi and H. Roshanfekr (2008). Effect of Ionophores Monensin and Lasalocid on Performance and Carcass Characteristics in Fattening Arabi Lambs. Pakistan Journal of Nutrition, 7(1): 81-84.

Hudd, D.L. (1983). Pharmacological Basis of Large Animal Medicine. Oxford, Mlack well, (107-128).

Jack, E.M.Et., R.J. Fallon., F.J. Harte and M.J. Drennan (1986). Effect of avoparcin, flavomycin and monensin sodium inclusion in a calf concentrate diet on calf performance. Irish Journal of Agricultrural Research 25: 197-204.

Kolankaya, D., G. Selmanoğlu., K. Sorkun and B. Salih (2002). Protective effects of Turkish propolis on alcohol-induced serum lipid changes and liver injury in male rats. Food Chemistry, 78, 213–217.

Marcucci, M.C., F. Ferreres., C. García-Viguera., V.S. Bankova., S.L. De Castro., A.P. Dantas., P.H.M. Valente and N. Paulino (2001). Phenolic compounds from Brazilian propolis with pharmacological activities. J. Ethnopharmacol. 74, 105–112.

Matabudul, D.K., B. Conway., I. Lumley and S. Sumar (2001). The simultaneous determination of the ionophore antibiotics in animal tissue and eggs by tandem electrospray LC-MS-MS. Food Chem., 75: 345-354.

Murray, P.J., J. B. Rowe and E. M. Aitcheson (1990). The influence of protein quality on the effect of flavomycin on wool growth, live weight change and rumen fermentation in sheep. Aust. J. Agric.

Ota, C., C. Unterkircher., V. Fantinato and M.T. Shimizu (2001). Antifungal activity of propolis on different species of candida. Mycoses, 44(9-10): 375-378.

Sarker, M.S.K and C.J. Yang (2010). Propolis and Illite as Feed Additives on Performance and Blood Profiles of Pre-Weaning Hanwoo Calves. Journal of Animal and Veterinary Advances. 9(19): 2526-2531.

SAS Institute Inc., (2004). Procedures Guide for Personal Computer, Statistical Analysis System Institute. Inc., Carry, N.C.

Sharifi, S.D.,   A. Dibamehr, H. Lotfollahian and B. Baurhoo (2012). Effects of flavomycin and probiotic supplementation to diets containing different sources of fat on growth performance, intestinal morphology, apparent metabolizable energy, and fat digestibility in broiler chickens.   Poultry Science (91):918–927.

Stangaciu, S (1999). Apitherapy Internet Course Notes, AIC; 1999. p. 257.

Thickett B., D. Mitchell and B. Hallows (1988). Calf Rearing. 2^nd ed. UK: Farming Press. LTD.

Valero, M.V., R.M. Prado., F. Zawadzki., C.E. Eiras., G.S. Madrona and I.N. Prado (2014). Propolis and essential oils additives in the diets improved animal performance and feed efficiency of bulls finished in feedlot. Acta Scientiarum Anim Sci., (36):419 –426. 

Van der Bergh, G. (1995). The use of growth promoters in beef production. Pro Agri 3, 22-24.

Van der Merwe, B., J., T.J. Dugmore and K.P. Walsh. (2001). The effect of flavophospholipol (Flavomycin®) on milk production and milk urea nitrogen concentrations of grazing dairy cows. South African Journal of Animal Science, 31(2): 101-106.

Vynograd, N., I. Vynograd and Z. Sosnowski (2000). A comparative multicentre study of the efficacy of propolis, acyclovir and placebo in the treatment of genital herpes (HSV). Phytomedicine, (7): 1-6.

Wang, N.Z and Li D. (2004). Effect of combined propolis-ethanol-extract and Shaoyao-Gancao-tang on blood sugar Levels in rabbits with alloxan induced experimental diabetes. Asia Pac J Clin Nutr.   13:S66.

Webster, J (1986). Calf husbandry, Health and welfare. London: Reprinted by Collins Proffessional and technical Books.

Yucel, B., A. Onenc., A. Kaya and O. Altan (2015). Effects of Propolis Administration on Growth Performance and Neonatal Diarrhea of Calves.  SOJ Veterinary Sciences, 1-5.

Ziaran, H.R., H.R. Rahmani., J. Pourreza (2005). Effect of dietary oil extract of propolis on immune response and broiler performance. Pakistan J. of Biological Sciences, Faisalabad 8 (10):1485.

Research :